ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (2): 381-387.doi: 10.11843/j.issn.0366-6964.2016.02.023

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The Role of MAPK Signaling Pathway in Quinestrol-induced Apoptosis of Spermatogenic Cells in Rat

LI Jian1* ,CHEN Yao-xing2,CHEN Fu-ning3,WANG Zi-xu2,CAO Jing2,DONG Yu-lan2   

  1. (1.College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471003, China;2.Laboratory of Veterinary Anatomy,College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;3.Changping Hospital of Integrated Chinese and Western Medicine,Changping,Beijing 102208,China)
  • Received:2015-09-08 Online:2016-02-23 Published:2016-02-23

Abstract:

This experiment was conducted to study the role of MAPK signaling pathway in quinestrol-induced apoptosis of spermatogenic cells in rat.Twenty 8-week-old male SD rats were randomly divided into 4 groups and were treated by using daily intraperitoneal injection of 0.00,0.01,0.10 and 1.00 mg•kg-1 of BW quinestrol dissolved in olive oil for 1 week after 1-week adaptive feeding.At the end of the treatment,the testis tissue sections were prepared and the morphology of the testis tissue was observed by HE staining.The immunohistochemistry staining was performed to measure the expression levels of PCNA,MAPK signaling pathway of p-ERK,p-JNK and p-P38 in spermatogenic cells and TUNEL method was used to detect apoptosis of spermatogenic cells.The results showed that the epithelial thickness of rat seminiferous tube decreased as well as the number of spermatogenic cells decreased.Numerous spermatocytes and spermatids showed swelling or shrinkage and meanwhile the number of mature sperm reduced in the lumen after quinestrol exposure.The expressions of PCNA and p-ERK in the spermatogenic cells were significantly decreased.In contrast,the expressions of TUNEL,p-JNK protein and p-P38 protein were significantly increased.The results suggest that quinestrol exposure inhibited the development of rat testis by inhibiting proliferation and promoting MAPK-mediated apoptosis of spermatogenic cells.

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